You want to determine the level of expression of a gene in a given condition.
A Real-time PCR is performed by BIOMNIGENE on the cDNA resulting from the reverse transcription of messenger RNAs/obtained by mRNA reverse transcrition (in order) to assess the level of expression of the target gene.
Your needs
Genetic material
Total RNA
Total messenger RNA
Target mRNA(s)
Methods
De Novo sequencing
Re-sequening
Analysis of splice variants
Techniques
NGS
Sanger
RT-qPCR