We accept all libraries compatible with illumina technology. Libraries can derive from genomic DNA for complete genome sequencing or from RNA for transcriptome studies. Ampicon libraries can also be analyzed.

To be compatible with Illumina technology, library DNA fragments must contain four components :

• flow-cell binding sites: sequences known as P5 and P7 allowing the binding of the fragments constituting the library on the flow-cell and DNA strand clustering.
• sequencing primer binding sites: sequences necessary to initiate sequencing from each extremity of the fragments of interest.
• index sequences: short sequences (6-8 bp) called i5 and i7 that serve as barcodes to allow sample multiplexing. The use of double indexing makes it possible to sequence a larger number of samples with a reduced number of unique indexes. For example, to sequence 96 single-index samples, 96 different indexes are required, whereas in the case of double indexing, only 20 different indexes are needed.
• primer hybridization sites allowing indexes to be read.

The combination of these elements constituting the so-called adapters may vary depending on the configuration in single or double index.

The customer have to inform Biomnigene if any of the above-mentioned components are modified or are missing, to adjust sequencing parameters.

Yes, we add Phix for free to your libraries for all sequencing performed on our devices.

The Phix library serves as an internal sequencing control and brings diversity to the libraries.

The amount of Phix library added depends on the nature of your library. For example for a genomic DNA library, we recommend only 5% of Phix, whereas for an amplicon library, we recommend 20 to 25% of Phix, because the diversity in this type of library is more limited.

Yes, we perform a capillary electrophoresis to check if the size of the library is homogeneous, as well as the quantification of the library by fluorimetry on Qubit.