{"id":659,"date":"2026-05-28T18:02:15","date_gmt":"2026-05-28T18:02:15","guid":{"rendered":"https:\/\/www.biomnigene.fr\/differential-expression\/"},"modified":"2026-09-10T13:00:52","modified_gmt":"2026-09-10T13:00:52","slug":"differential-expression","status":"publish","type":"page","link":"https:\/\/www.biomnigene.fr\/en\/differential-expression\/","title":{"rendered":"Differential Expression"},"content":{"rendered":"<div class=\"et_pb_section_0 et_pb_section et_section_regular et_flex_section\">\n<div class=\"et_pb_row_0 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_0 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n<div class=\"et_pb_module et_d4_element dsm_breadcrumbs dsm_breadcrumbs_0\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module_inner\">\n\t\t\t\t\t<nav class=\"dsm_breadcrumbs_wrap\">\n\t\t\t\t<ul class=\"dsm_breadcrumbs\" itemscope itemtype=\"https:\/\/schema.org\/BreadcrumbList\">\t\t\t<li class=\"dsm_breadcrumbs_item dsm_home_item dsm_home_item_custom\" itemprop=\"itemListElement\" itemscope\n\t\t\titemtype=\"https:\/\/schema.org\/ListItem\">\n\t\t\t\t<a href=\"https:\/\/www.biomnigene.fr\/en\/\" itemprop=\"item\" target=\"\">\n\t\t\t\t\t<span class='dsm_home_text_wrapper'>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"et-pb-icon dsm_home_icon\">\ue074<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"dsm_home_text\" itemprop=\"name\">\n\t\t\t\t\t\t\t\t \t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t<\/a>\n\t\t\t\t<meta itemprop=\"position\" content=\"1\" \/>\n\t\t\t<\/li>\n\n\t\t<li class=\"dsm_breadcrumbs_separator\"> <span class=\"et-pb-icon dsm_separator_icon\">5<\/span><\/li><\/ul>\n\t\t\t<\/nav>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n<\/div>\n<\/div>\n\n<div class=\"et_pb_row_1 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_1 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_0 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module preset--module--divi-text--default\"><div class=\"et_pb_text_inner\"><h1>Differential Expression<\/h1>\n<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_section_1 et_pb_section et_section_regular et_flex_section\">\n<div class=\"et_pb_row_2 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_2 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_1 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>Compare Gene Expression Between Different Conditions<\/strong><\/h2>\n<\/div><\/div>\n<\/div>\n\n<div class=\"et_pb_column_3 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_2 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>Differential expression analysis identifies <strong>variations in the expression of one or more genes between different biological or experimental conditions.<\/strong> It involves measuring the amount of RNA associated with each gene and comparing these expression levels across multiple samples.<\/p>\n<\/div><\/div>\n<\/div>\n\n<div class=\"et_pb_column_4 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_3 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>This approach is widely used to understand how an organism reacts to a change in condition: exposure to a molecule, environmental modification, difference between two tissues, or comparison between several organisms.<\/p>\n<p>The study of these variations notably highlights genes whose activity increases or decreases in a given situation.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_row_3 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_5 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_image_0 et_pb_image et_pb_module et_flex_module\"><span class=\"et_pb_image_wrap\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-expression-differentielle-1.webp\" title=\"img-expression-differentielle-1\" width=\"1700\" height=\"567\" srcset=\"https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-expression-differentielle-1.webp 1700w, https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-expression-differentielle-1-1280x427.webp 1280w, https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-expression-differentielle-1-980x327.webp 980w, https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-expression-differentielle-1-480x160.webp 480w\" sizes=\"(min-width: 0px) and (max-width: 480px) 480px, (min-width: 481px) and (max-width: 980px) 980px, (min-width: 981px) and (max-width: 1280px) 1280px, (min-width: 1281px) 1700px, 100vw\" class=\"wp-image-374\" \/><\/span><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_row_4 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_6 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_4 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>Why Analyze Differential Expression?<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_5 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>Comparing gene expression levels helps to better understand the biological mechanisms involved in a studied phenomenon.<\/p>\n<p>Differential expression analysis is notably used to:<\/p>\n<ul>\n<li>identify genes involved in a specific biological response<\/li>\n<li>study the impact of a treatment or an environmental factor<\/li>\n<li>compare organisms or strains cultured under the same conditions<\/li>\n<li>analyze differences between tissues, developmental stages, or physiological states<\/li>\n<li>identify genes potentially associated with a particular biological process.<\/li>\n<\/ul>\n<p>This approach is an essential tool for exploring the molecular mechanisms underlying a biological phenomenon.<\/p>\n<\/div><\/div>\n\n<div class=\"et_pb_module et_pb_button_module_wrapper et_pb_button_0_wrapper preset--module--divi-button--ssmr0y6bs7_wrapper\"><a class=\"et_pb_button_0 et_pb_button et_pb_bg_layout_dark et_pb_module et_block_module preset--module--divi-button--ssmr0y6bs7\" href=\"https:\/\/www.biomnigene.fr\/contact\/\" data-icon=\"$\">Contact Us<\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_section_2 et_pb_section et_section_regular et_flex_section\">\n<div class=\"et_pb_row_5 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_7 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_6 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: center;\"><strong>Techniques Used<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_7 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align: center;\">Several methods can be employed to analyze differential gene expression.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_row_6 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_8 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_group_0 et_pb_group et-last-child et_pb_module et_flex_group et_pb_css_mix_blend_mode_passthrough\">\n<div class=\"et_pb_text_8 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h3 style=\"text-align: left;\"><strong>RT-qPCR<\/strong><\/h3>\n<\/div><\/div>\n\n<div class=\"et_pb_text_9 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>Real-time quantitative PCR (RT-qPCR) precisely measures the amount of cDNA corresponding to a gene of interest. This technique monitors the amplification of a genetic sequence during PCR cycles using a fluorescent signal proportional to the amount of amplified DNA.  <\/p>\n<p>It notably allows for:<\/p>\n<ul>\n<li>comparing gene expression between several samples<\/li>\n<li>precisely measuring expression variations<\/li>\n<li>analyzing multiple genes in the same study.<\/li>\n<\/ul>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_column_9 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_group_1 et_pb_group et-last-child et_pb_module et_flex_group et_pb_css_mix_blend_mode_passthrough\">\n<div class=\"et_pb_text_10 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h3 style=\"text-align: left;\"><strong>Transcriptomic Sequencing (RNA-Seq)<\/strong><\/h3>\n<\/div><\/div>\n\n<div class=\"et_pb_text_11 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p><strong>High-throughput sequencing<\/strong> technologies allow for the analysis of the expression of thousands of genes simultaneously. Expression is estimated by counting RNA fragments corresponding to each gene in the studied samples.  <\/p>\n<p>This approach offers a global view of an organism's genetic activity.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_column_10 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_group_2 et_pb_group et-last-child et_pb_module et_flex_group et_pb_css_mix_blend_mode_passthrough\">\n<div class=\"et_pb_text_12 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h3 style=\"text-align: left;\"><strong>Digital PCR (dPCR)<\/strong><\/h3>\n<\/div><\/div>\n\n<div class=\"et_pb_text_13 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p><strong>Digital PCR<\/strong> enables absolute quantification of nucleic acids, without a standard curve or reference sample. The sample is divided into several thousand individual and independent reactions, which provides high tolerance to inhibitors, superior precision, increased sensitivity, and excellent reproducibility. <\/p>\n<p>It is particularly suitable for:<\/p>\n<ul>\n<li>determining the copy number of a given DNA target<\/li>\n<li>detecting rare mutations<\/li>\n<li>measuring the viral load of a sample<\/li>\n<li>accurately analyzing gene expression differences<\/li>\n<li>quantifying a high-throughput sequencing library<\/li>\n<\/ul>\n<p>Biomnig\u00e8ne is equipped with the <strong>QuantStudio Absolute Q<\/strong> plate digital PCR system.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_column_11 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_14 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_section_3 et_pb_section et_section_regular et_flex_section\">\n<div class=\"et_pb_row_7 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_12 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_group_3 et_pb_group et-last-child et_pb_module et_flex_group et_pb_css_mix_blend_mode_passthrough\">\n<div class=\"et_pb_text_15 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>Interpretation of Results<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_16 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>Differential expression analysis identifies genes referred to as <strong>differentially expressed<\/strong>, meaning their expression level varies significantly between two or more conditions.<\/p>\n<p>These genes can be:<\/p>\n<ul>\n<li><strong>overexpressed<\/strong> in a given condition<\/li>\n<li><strong>underexpressed<\/strong> compared to a reference condition.<\/li>\n<\/ul>\n<p>Identifying these variations provides valuable information for understanding the biological mechanisms involved in an organism's response.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_column_13 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_group_4 et_pb_group et-last-child et_pb_module et_flex_group et_pb_css_mix_blend_mode_passthrough\">\n<div class=\"et_pb_text_17 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>An Analysis Tailored to Your Project<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_18 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>The study of differential expression can be applied to numerous scientific fields:<\/p>\n<ul>\n<li>fundamental research<\/li>\n<li>cell and molecular biology<\/li>\n<li>microbiology<\/li>\n<li>biotechnologies<\/li>\n<li>medical research.<\/li>\n<\/ul>\n<p>Depending on the nature of the samples and the objectives of the study, different analytical strategies can be implemented to obtain the most relevant results.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_section_4 et_pb_section et_section_regular et_flex_section\"><div class=\"et_pb_row_8 et_pb_row et_flex_row preset--module--divi-row--default\"><div class=\"et_pb_column_14 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\"><div class=\"et_pb_text_19 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align:center;\"><strong>Int\u00e9ress\u00e9 par notre offre ?<\/strong><\/h2>\n<\/div><\/div><div class=\"et_pb_text_20 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:center;\">Vous souhaitez des informations compl\u00e9mentaires ?<\/p>\n<\/div><\/div><div class=\"et_pb_module et_pb_button_module_wrapper et_pb_button_1_wrapper preset--module--divi-button--6hgv0h8r0g_wrapper\"><a class=\"et_pb_button_1 et_pb_button et_pb_bg_layout_dark et_pb_module et_block_module preset--module--divi-button--6hgv0h8r0g\" href=\"https:\/\/www.biomnigene.fr\/contact\/\" data-icon=\"$\">Nous contacter<\/a><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-659","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Differential Gene Expression Analysis | Biomnig\u00e8ne<\/title>\n<meta name=\"description\" content=\"Identify differentially expressed genes between your conditions with Biomnig\u00e8ne. 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