{"id":647,"date":"2026-05-28T18:27:10","date_gmt":"2026-05-28T18:27:10","guid":{"rendered":"https:\/\/www.biomnigene.fr\/exon-determination-alternative-splicing\/"},"modified":"2026-09-10T13:00:04","modified_gmt":"2026-09-10T13:00:04","slug":"exon-determination-alternative-splicing","status":"publish","type":"page","link":"https:\/\/www.biomnigene.fr\/en\/exon-determination-alternative-splicing\/","title":{"rendered":"Exon Determination \/ Alternative Splicing"},"content":{"rendered":"<div class=\"et_pb_section_0 et_pb_section et_section_regular et_flex_section\">\n<div class=\"et_pb_row_0 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_0 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n<div class=\"et_pb_module et_d4_element dsm_breadcrumbs dsm_breadcrumbs_0\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module_inner\">\n\t\t\t\t\t<nav class=\"dsm_breadcrumbs_wrap\">\n\t\t\t\t<ul class=\"dsm_breadcrumbs\" itemscope itemtype=\"https:\/\/schema.org\/BreadcrumbList\">\t\t\t<li class=\"dsm_breadcrumbs_item dsm_home_item dsm_home_item_custom\" itemprop=\"itemListElement\" itemscope\n\t\t\titemtype=\"https:\/\/schema.org\/ListItem\">\n\t\t\t\t<a href=\"https:\/\/www.biomnigene.fr\/en\/\" itemprop=\"item\" target=\"\">\n\t\t\t\t\t<span class='dsm_home_text_wrapper'>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"et-pb-icon dsm_home_icon\">\ue074<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"dsm_home_text\" itemprop=\"name\">\n\t\t\t\t\t\t\t\t \t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t<\/a>\n\t\t\t\t<meta itemprop=\"position\" content=\"1\" \/>\n\t\t\t<\/li>\n\n\t\t<li class=\"dsm_breadcrumbs_separator\"> <span class=\"et-pb-icon dsm_separator_icon\">5<\/span><\/li><\/ul>\n\t\t\t<\/nav>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n<\/div>\n<\/div>\n\n<div class=\"et_pb_row_1 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_1 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_0 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module preset--module--divi-text--default\"><div class=\"et_pb_text_inner\"><h1>Exon Determination \/ Alternative Splicing<\/h1>\n<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_section_1 et_pb_section et_section_regular et_flex_section\">\n<div class=\"et_pb_row_2 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_2 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_1 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>Identifying Coding Regions and Splicing Variants of a Gene<\/strong><\/h2>\n<\/div><\/div>\n<\/div>\n\n<div class=\"et_pb_column_3 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_2 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>Analysis of gene structure enables precise determination of <strong>exons<\/strong>, i.e., the coding regions retained in mature messenger RNA. This step is essential for understanding the organization of a gene and how it is expressed in an organism. <\/p>\n<p>In many organisms, a single gene can produce several distinct messenger RNAs through a mechanism called <strong>alternative splicing.<\/strong><\/p>\n<\/div><\/div>\n<\/div>\n\n<div class=\"et_pb_column_4 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_3 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>This process involves assembling coding segments (exons) differently during RNA maturation, thereby generating different versions of RNA and thus proteins from a single gene. <\/p>\n<p>The study of these variations constitutes an important tool for understanding the regulation of gene expression and the functional diversity of proteins.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_row_3 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_5 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_image_0 et_pb_image et_pb_module et_flex_module\"><span class=\"et_pb_image_wrap\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-determination-des-exons-1.webp\" title=\"img-determination-des-exons-1\" width=\"1700\" height=\"567\" srcset=\"https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-determination-des-exons-1.webp 1700w, https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-determination-des-exons-1-1280x427.webp 1280w, https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-determination-des-exons-1-980x327.webp 980w, https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-determination-des-exons-1-480x160.webp 480w\" sizes=\"(min-width: 0px) and (max-width: 480px) 480px, (min-width: 481px) and (max-width: 980px) 980px, (min-width: 981px) and (max-width: 1280px) 1280px, (min-width: 1281px) 1700px, 100vw\" class=\"wp-image-388\" \/><\/span><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_row_4 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_6 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_4 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>Why Study Exons and Alternative Splicing?<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_5 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>Characterization of exons and splicing variants enables exploration of several aspects of a gene's molecular biology:<\/p>\n<ul>\n<li>identify coding regions involved in protein production<\/li>\n<li>understand the transcriptional structure of a gene<\/li>\n<li>detect different transcripts arising from a single genetic locus<\/li>\n<li>analyze the regulation of gene expression according to tissues or experimental conditions<\/li>\n<li>study molecular mechanisms that may lead to different functional variants.<\/li>\n<\/ul>\n<p>Alternative splicing plays a central role in the diversity of proteins produced by an organism, enabling the generation of multiple isoforms from a single gene.<\/p>\n<\/div><\/div>\n<\/div>\n\n<div class=\"et_pb_column_7 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_6 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>Analysis Principle<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_7 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>Identification of exons and splicing variants is generally based on the study of <strong>RNAs transcribed from a gene.<\/strong><\/p>\n<p>After RNA extraction, it can be converted into complementary DNA (cDNA), then analyzed to:<\/p>\n<ul>\n<li>determine the <strong>exon-exon junctions<\/strong> present in transcripts<\/li>\n<li>identify the <strong>messenger RNA isoforms<\/strong> produced by a gene<\/li>\n<li>compare splicing profiles between different biological conditions.<\/li>\n<\/ul>\n<p>These analyses provide better understanding of how a gene can produce multiple transcripts and how these variants are regulated.<\/p>\n<\/div><\/div>\n\n<div class=\"et_pb_module et_pb_button_module_wrapper et_pb_button_0_wrapper preset--module--divi-button--ssmr0y6bs7_wrapper\"><a class=\"et_pb_button_0 et_pb_button et_pb_bg_layout_dark et_pb_module et_block_module preset--module--divi-button--ssmr0y6bs7\" href=\"https:\/\/www.biomnigene.fr\/contact\/\" data-icon=\"$\">Contact Us<\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_section_2 et_pb_section et_section_regular et_flex_section\">\n<div class=\"et_pb_row_5 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_8 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_8 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: center;\"><strong>Methods Used to Analyze Splicing<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_9 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align: center;\">Several analytical approaches can be implemented to characterize exons and splicing variants.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_row_6 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_9 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_group_0 et_pb_group et-last-child et_pb_module et_flex_group et_pb_css_mix_blend_mode_passthrough\">\n<div class=\"et_pb_text_10 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h3 style=\"text-align: left;\"><strong>NGS Sequencing<\/strong><\/h3>\n<\/div><\/div>\n\n<div class=\"et_pb_text_11 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p><strong>Next-generation sequencing (NGS)<\/strong> enables simultaneous analysis of a large number of RNA fragments. This approach provides a detailed view of transcript structure and enables identification of different splicing events. <\/p>\n<p>It is particularly suited for:<br \/>detecting unknown RNA isoforms<br \/>precisely mapping exons<br \/>analyzing splicing variants at the transcriptome scale.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_column_10 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_group_1 et_pb_group et-last-child et_pb_module et_flex_group et_pb_css_mix_blend_mode_passthrough\">\n<div class=\"et_pb_text_12 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h3 style=\"text-align: left;\"><strong>Targeted Sequencing<\/strong><\/h3>\n<\/div><\/div>\n\n<div class=\"et_pb_text_13 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>In certain cases, targeted analysis can be performed to study a specific gene or region. <strong>Sanger sequencing<\/strong> then enables confirmation of transcript structure or validation of certain splicing variants. <\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_column_11 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_group_2 et_pb_group et-last-child et_pb_module et_flex_group et_pb_css_mix_blend_mode_passthrough\">\n<div class=\"et_pb_text_14 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h3 style=\"text-align: left;\"><strong>Validation by RT-qPCR<\/strong><\/h3>\n<\/div><\/div>\n\n<div class=\"et_pb_text_15 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p><strong>RT-qPCR<\/strong> can also be used to measure the relative abundance of different RNA isoforms or compare their expression under multiple experimental conditions.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_section_3 et_pb_section et_section_regular et_flex_section\">\n<div class=\"et_pb_row_7 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_12 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_group_3 et_pb_group et-last-child et_pb_module et_flex_group et_pb_css_mix_blend_mode_passthrough\">\n<div class=\"et_pb_text_16 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>Types of Genetic Material Analyzed<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_17 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>Splicing analyses are primarily based on the study of different types of RNA:<\/p>\n<ul>\n<li>total RNA<\/li>\n<li>total messenger RNA<\/li>\n<li>targeted mRNA corresponding to a specific gene.<\/li>\n<\/ul>\n<p>The choice of material depends on the scientific objective and the level of precision desired in the study of splicing variants.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_column_13 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_group_4 et_pb_group et-last-child et_pb_module et_flex_group et_pb_css_mix_blend_mode_passthrough\">\n<div class=\"et_pb_text_18 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>An Approach Tailored to Each Scientific Project<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_19 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>The study of the effect of active molecules or external factors on gene expression can be applied to many fields:<\/p>\n<ul>\n<li>molecular biology<\/li>\n<li>microbiology<\/li>\n<li>medical research<\/li>\n<li>biotechnology<\/li>\n<li>environmental studies.<\/li>\n<\/ul>\n<p>Analytical strategies can be adapted according to sample type, number of genes studied, and desired precision.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_section_4 et_pb_section et_section_regular et_flex_section\"><div class=\"et_pb_row_8 et_pb_row et_flex_row preset--module--divi-row--default\"><div class=\"et_pb_column_14 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\"><div class=\"et_pb_text_20 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align:center;\"><strong>Int\u00e9ress\u00e9 par notre offre ?<\/strong><\/h2>\n<\/div><\/div><div class=\"et_pb_text_21 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:center;\">Vous souhaitez des informations compl\u00e9mentaires ?<\/p>\n<\/div><\/div><div class=\"et_pb_module et_pb_button_module_wrapper et_pb_button_1_wrapper preset--module--divi-button--6hgv0h8r0g_wrapper\"><a class=\"et_pb_button_1 et_pb_button et_pb_bg_layout_dark et_pb_module et_block_module preset--module--divi-button--6hgv0h8r0g\" href=\"https:\/\/www.biomnigene.fr\/contact\/\" data-icon=\"$\">Nous contacter<\/a><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-647","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Alternative Splicing and Exon Determination | Biomnig\u00e8ne<\/title>\n<meta name=\"description\" content=\"Identify exons and alternative splicing variants of your genes with Biomnig\u00e8ne. 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