{"id":643,"date":"2026-05-28T18:16:30","date_gmt":"2026-05-28T18:16:30","guid":{"rendered":"https:\/\/www.biomnigene.fr\/determine-the-effect-of-active-compounds-or-external-factors-on-gene-expression\/"},"modified":"2026-09-10T12:59:54","modified_gmt":"2026-09-10T12:59:54","slug":"determine-the-effect-of-active-compounds-or-external-factors-on-gene-expression","status":"publish","type":"page","link":"https:\/\/www.biomnigene.fr\/en\/determine-the-effect-of-active-compounds-or-external-factors-on-gene-expression\/","title":{"rendered":"Determine the effect of active compounds or external factors on gene expression"},"content":{"rendered":"<div class=\"et_pb_section_0 et_pb_section et_section_regular et_flex_section\">\n<div class=\"et_pb_row_0 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_0 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n<div class=\"et_pb_module et_d4_element dsm_breadcrumbs dsm_breadcrumbs_0\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module_inner\">\n\t\t\t\t\t<nav class=\"dsm_breadcrumbs_wrap\">\n\t\t\t\t<ul class=\"dsm_breadcrumbs\" itemscope itemtype=\"https:\/\/schema.org\/BreadcrumbList\">\t\t\t<li class=\"dsm_breadcrumbs_item dsm_home_item dsm_home_item_custom\" itemprop=\"itemListElement\" itemscope\n\t\t\titemtype=\"https:\/\/schema.org\/ListItem\">\n\t\t\t\t<a href=\"https:\/\/www.biomnigene.fr\/en\/\" itemprop=\"item\" target=\"\">\n\t\t\t\t\t<span class='dsm_home_text_wrapper'>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"et-pb-icon dsm_home_icon\">\ue074<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"dsm_home_text\" itemprop=\"name\">\n\t\t\t\t\t\t\t\t \t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t<\/a>\n\t\t\t\t<meta itemprop=\"position\" content=\"1\" \/>\n\t\t\t<\/li>\n\n\t\t<li class=\"dsm_breadcrumbs_separator\"> <span class=\"et-pb-icon dsm_separator_icon\">5<\/span><\/li><\/ul>\n\t\t\t<\/nav>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n<\/div>\n<\/div>\n\n<div class=\"et_pb_row_1 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_1 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_0 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module preset--module--divi-text--default\"><div class=\"et_pb_text_inner\"><h1>Determine the effect of active compounds or external factors on gene expression<\/h1>\n<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_section_1 et_pb_section et_section_regular et_flex_section\">\n<div class=\"et_pb_row_2 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_2 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_1 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>Study the impact of an environment on gene activity<\/strong><\/h2>\n<\/div><\/div>\n<\/div>\n\n<div class=\"et_pb_column_3 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_2 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>In many research projects, it is essential to understand how an <strong>external factor<\/strong> influences gene expression. An active compound, a pharmacological treatment, environmental stress, or an experimental modification can alter an organism\u2019s transcriptional activity. <\/p>\n<\/div><\/div>\n<\/div>\n\n<div class=\"et_pb_column_4 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_3 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>Studying these variations is based on the analysis of <strong>RNAs produced during gene transcription.<\/strong> By comparing expression levels across different experimental conditions, it becomes possible to identify genes whose activity is modulated by the factor under study. <\/p>\n<p>This approach makes it possible to explore the molecular mechanisms involved in an organism\u2019s biological response.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_row_3 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_5 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_image_0 et_pb_image et_pb_module et_flex_module\"><span class=\"et_pb_image_wrap\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-determiner-effet-de-molecules-actives-1.webp\" title=\"img-determiner-effet-de-molecules-actives-1\" width=\"1700\" height=\"567\" srcset=\"https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-determiner-effet-de-molecules-actives-1.webp 1700w, https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-determiner-effet-de-molecules-actives-1-1280x427.webp 1280w, https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-determiner-effet-de-molecules-actives-1-980x327.webp 980w, https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-determiner-effet-de-molecules-actives-1-480x160.webp 480w\" sizes=\"(min-width: 0px) and (max-width: 480px) 480px, (min-width: 481px) and (max-width: 980px) 980px, (min-width: 981px) and (max-width: 1280px) 1280px, (min-width: 1281px) 1700px, 100vw\" class=\"wp-image-380\" \/><\/span><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_row_4 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_6 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_4 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>Why analyse the effect of a factor on gene expression?<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_5 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>Gene expression analysis makes it possible to observe an organism\u2019s response to external stimulation. It is frequently used to: <\/p>\n<ul>\n<li>analyse the effect of a drug or chemical compound on the activity of certain genes<\/li>\n<li>understand an organism\u2019s molecular response to environmental stress<\/li>\n<li>study the impact of an experimental treatment on the expression of a panel of genes<\/li>\n<li>identify biological pathways activated or inhibited under a given condition.<\/li>\n<\/ul>\n<p>These analyses make it possible to link an observable biological phenomenon to specific changes in gene expression.<\/p>\n<\/div><\/div>\n<\/div>\n\n<div class=\"et_pb_column_7 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_6 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>Principle of the analysis<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_7 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>Studying the effect of a molecule or an external factor generally relies on comparing several experimental conditions:<\/p>\n<ul>\n<li>a reference sample (control condition)<\/li>\n<li>one or more samples exposed to the factor under study.<\/li>\n<\/ul>\n<p>After RNA extraction, it is converted into <strong>complementary DNA (cDNA)<\/strong> for analysis. Gene expression levels can then be compared across the different experimental conditions to detect exposure-induced variations. <\/p>\n<\/div><\/div>\n\n<div class=\"et_pb_module et_pb_button_module_wrapper et_pb_button_0_wrapper preset--module--divi-button--ssmr0y6bs7_wrapper\"><a class=\"et_pb_button_0 et_pb_button et_pb_bg_layout_dark et_pb_module et_block_module preset--module--divi-button--ssmr0y6bs7\" href=\"https:\/\/www.biomnigene.fr\/contact\/\" data-icon=\"$\">Contact us<\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_section_2 et_pb_section et_section_regular et_flex_section\">\n<div class=\"et_pb_row_5 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_8 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_8 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: center;\"><strong>Possible analysis methods<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_9 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align: center;\">The choice of analytical strategy depends on the number of genes studied and the project\u2019s scientific objectives.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_row_6 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_9 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_group_0 et_pb_group et-last-child et_pb_module et_flex_group et_pb_css_mix_blend_mode_passthrough\">\n<div class=\"et_pb_text_10 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h3 style=\"text-align: left;\"><strong>Targeted analysis by RT-qPCR<\/strong><\/h3>\n<\/div><\/div>\n\n<div class=\"et_pb_text_11 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p><strong>Real-time quantitative PCR (RT-qPCR)<\/strong> makes it possible to precisely measure the expression level of a gene of interest. This method amplifies a specific genetic sequence and measures, in real time, the amount of amplified product using a fluorescent signal.  <\/p>\n<p>It is particularly suitable when the study focuses on:<br \/>a specific gene<br \/>a small number of targeted genes<br \/>validation of results obtained from more global analyses.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_column_10 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_group_1 et_pb_group et-last-child et_pb_module et_flex_group et_pb_css_mix_blend_mode_passthrough\">\n<div class=\"et_pb_text_12 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h3 style=\"text-align: left;\"><strong>Transcriptome sequencing (RNA-Seq)<\/strong><\/h3>\n<\/div><\/div>\n\n<div class=\"et_pb_text_13 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>When the objective is to explore the impact of a factor on a large number of genes, it is possible to perform <strong>transcriptome sequencing (RNA-Seq).<\/strong><\/p>\n<p>This approach makes it possible to:<\/p>\n<ul>\n<li>identify the genes expressed in the sample<\/li>\n<li>measure their expression level<\/li>\n<li>detect expression changes at the transcriptome scale.<\/li>\n<\/ul>\n<p>It therefore provides a global view of an organism\u2019s genetic response.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_column_11 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_14 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_section_3 et_pb_section et_section_regular et_flex_section\">\n<div class=\"et_pb_row_7 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_12 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_group_2 et_pb_group et-last-child et_pb_module et_flex_group et_pb_css_mix_blend_mode_passthrough\">\n<div class=\"et_pb_text_15 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>Genetic material analysed<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_16 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>Depending on the study objectives, different types of genetic material may be used:<\/p>\n<ul>\n<li>total RNA<\/li>\n<li>total messenger RNA<\/li>\n<li>targeted mRNAs corresponding to genes of interest.<\/li>\n<\/ul>\n<p>The choice of RNA type depends on the type of analysis and the desired level of precision.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_column_13 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_group_3 et_pb_group et-last-child et_pb_module et_flex_group et_pb_css_mix_blend_mode_passthrough\">\n<div class=\"et_pb_text_17 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>An approach tailored to each scientific project<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_18 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>Studying the effect of active compounds or external factors on gene expression can be applied to many fields:<\/p>\n<ul>\n<li>molecular biology<\/li>\n<li>microbiology<\/li>\n<li>medical research<\/li>\n<li>biotechnologies<\/li>\n<li>environmental studies.<\/li>\n<\/ul>\n<p>Analytical strategies can be adapted depending on the sample type, the number of genes studied, and the level of precision required.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_section_4 et_pb_section et_section_regular et_flex_section\"><div class=\"et_pb_row_8 et_pb_row et_flex_row preset--module--divi-row--default\"><div class=\"et_pb_column_14 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\"><div class=\"et_pb_text_19 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align:center;\"><strong>Int\u00e9ress\u00e9 par notre offre ?<\/strong><\/h2>\n<\/div><\/div><div class=\"et_pb_text_20 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:center;\">Vous souhaitez des informations compl\u00e9mentaires ?<\/p>\n<\/div><\/div><div class=\"et_pb_module et_pb_button_module_wrapper et_pb_button_1_wrapper preset--module--divi-button--6hgv0h8r0g_wrapper\"><a class=\"et_pb_button_1 et_pb_button et_pb_bg_layout_dark et_pb_module et_block_module preset--module--divi-button--6hgv0h8r0g\" href=\"https:\/\/www.biomnigene.fr\/contact\/\" data-icon=\"$\">Nous contacter<\/a><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-643","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Impact of active compounds on gene expression | Biomnig\u00e8ne<\/title>\n<meta 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