{"id":617,"date":"2026-05-26T21:21:51","date_gmt":"2026-05-26T21:21:51","guid":{"rendered":"https:\/\/www.biomnigene.fr\/researching-mutations-insertions-and-deletions\/"},"modified":"2026-09-10T12:58:46","modified_gmt":"2026-09-10T12:58:46","slug":"researching-mutations-insertions-and-deletions","status":"publish","type":"page","link":"https:\/\/www.biomnigene.fr\/en\/researching-mutations-insertions-and-deletions\/","title":{"rendered":"Researching mutations, insertions, and deletions"},"content":{"rendered":"<div class=\"et_pb_section_0 et_pb_section et_section_regular et_flex_section\">\n<div class=\"et_pb_row_0 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_0 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n\n\n\n\n\n\n\n<div class=\"et_pb_module et_d4_element dsm_breadcrumbs dsm_breadcrumbs_0\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module_inner\">\n\t\t\t\t\t<nav class=\"dsm_breadcrumbs_wrap\">\n\t\t\t\t<ul class=\"dsm_breadcrumbs\" itemscope itemtype=\"https:\/\/schema.org\/BreadcrumbList\">\t\t\t<li class=\"dsm_breadcrumbs_item dsm_home_item dsm_home_item_custom\" itemprop=\"itemListElement\" itemscope\n\t\t\titemtype=\"https:\/\/schema.org\/ListItem\">\n\t\t\t\t<a href=\"https:\/\/www.biomnigene.fr\/en\/\" itemprop=\"item\" target=\"\">\n\t\t\t\t\t<span class='dsm_home_text_wrapper'>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"et-pb-icon dsm_home_icon\">\ue074<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"dsm_home_text\" itemprop=\"name\">\n\t\t\t\t\t\t\t\t \t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t<\/a>\n\t\t\t\t<meta itemprop=\"position\" content=\"1\" \/>\n\t\t\t<\/li>\n\n\t\t<li class=\"dsm_breadcrumbs_separator\"> <span class=\"et-pb-icon dsm_separator_icon\">5<\/span><\/li><\/ul>\n\t\t\t<\/nav>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\n\n\n\n\n\n\n<\/div>\n<\/div>\n\n<div class=\"et_pb_row_1 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_1 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_0 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module preset--module--divi-text--default\"><div class=\"et_pb_text_inner\"><h1>Researching mutations, insertions, and deletions<\/h1>\n<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_section_1 et_pb_section et_section_regular et_flex_section\">\n<div class=\"et_pb_row_2 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_2 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_1 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>Identifying variations in a genetic sequence<\/strong><\/h2>\n<\/div><\/div>\n<\/div>\n\n<div class=\"et_pb_column_3 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_2 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>Genetic sequence analysis allows for the detection of <strong>modifications that have appeared in DNA<\/strong> over time. These variations can take different forms: point mutations, insertions, or deletions of DNA fragments. <\/p>\n<\/div><\/div>\n<\/div>\n\n<div class=\"et_pb_column_4 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_3 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>Researching these variations is essential for understanding the evolution of a genome, characterizing a genetic modification, or analyzing the differences between several sequences. <\/p>\n<p>Depending on the project objective, the analysis may focus on a <strong>specific gene<\/strong>, a DNA fragment, or an entire genome.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_row_3 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_5 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_image_0 et_pb_image et_pb_module et_flex_module\"><span class=\"et_pb_image_wrap\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-determiner-ou-confirmer-la-sequence-d-un-gene-ou-d-un-genome-entier-illustration-11-5.webp\" title=\"img-determiner-ou-confirmer-la-sequence-d-un-gene-ou-d-un-genome-entier-illustration-11\" width=\"1920\" height=\"640\" srcset=\"https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-determiner-ou-confirmer-la-sequence-d-un-gene-ou-d-un-genome-entier-illustration-11-5.webp 1920w, https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-determiner-ou-confirmer-la-sequence-d-un-gene-ou-d-un-genome-entier-illustration-11-5-1280x427.webp 1280w, https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-determiner-ou-confirmer-la-sequence-d-un-gene-ou-d-un-genome-entier-illustration-11-5-980x327.webp 980w, https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-determiner-ou-confirmer-la-sequence-d-un-gene-ou-d-un-genome-entier-illustration-11-5-480x160.webp 480w\" sizes=\"(min-width: 0px) and (max-width: 480px) 480px, (min-width: 481px) and (max-width: 980px) 980px, (min-width: 981px) and (max-width: 1280px) 1280px, (min-width: 1281px) 1920px, 100vw\" class=\"wp-image-294\" \/><\/span><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_row_4 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_6 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_4 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>When should you search for mutations or indels?<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_5 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>Researching mutations, insertions, or deletions can be useful in various scientific contexts, notably to:<\/p>\n<ul>\n<li>identify <strong>genetic variations that have appeared over time,<\/strong><\/li>\n<li>compare a genome to a <strong>reference sequence,<\/strong><\/li>\n<li>analyze modifications present in a <strong>target gene,<\/strong><\/li>\n<li>detect insertions or deletions in a specific genomic region.<\/li>\n<\/ul>\n<p>These analyses highlight the <strong>structural<\/strong> or <strong>point<\/strong> variations present in a DNA sequence.<\/p>\n<\/div><\/div>\n\n<div class=\"et_pb_module et_pb_button_module_wrapper et_pb_button_0_wrapper preset--module--divi-button--ssmr0y6bs7_wrapper\"><a class=\"et_pb_button_0 et_pb_button et_pb_bg_layout_dark et_pb_module et_block_module preset--module--divi-button--ssmr0y6bs7\" href=\"https:\/\/www.biomnigene.fr\/contact\/\" data-icon=\"$\">Contact us<\/a><\/div>\n<\/div>\n\n<div class=\"et_pb_column_7 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_6 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>Choosing the right strategy for your project<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_7 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p>Detecting mutations, insertions, or deletions requires choosing the analysis method best suited to the scientific context.<\/p>\n<p>Several parameters can influence this choice:<\/p>\n<ul>\n<li>the size of the genetic region studied,<\/li>\n<li>the type of variation sought,<\/li>\n<li>prior knowledge of<\/li>\n<li>the target sequence,<\/li>\n<li>the expected level of precision.<\/li>\n<\/ul>\n<p>BIOMNIGENE's technical teams support each project to determine the most relevant methodological approach.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_section_2 et_pb_section et_section_regular et_flex_section\">\n<div class=\"et_pb_row_5 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_8 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_8 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align:center;\"><strong>Which approaches to detect or quantify a g\u00e8ne?<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_9 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:center;\">The choice of method depends on the study objective and the extent of the genetic region analyzed.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_row_6 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_9 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_group_0 et_pb_group et-last-child et_pb_module et_flex_group et_pb_css_mix_blend_mode_passthrough\">\n<div class=\"et_pb_text_10 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h3 style=\"text-align:left;\"><strong>Targeted gene sequencing<\/strong><\/h3>\n<\/div><\/div>\n\n<div class=\"et_pb_text_11 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:left;\">When a mutation is sought in a <strong>precise region of the genome<\/strong>, targeted sequencing can be performed on the gene of interest.<\/p>\n<p><strong>Sanger sequencing<\/strong> is particularly suitable for analyzing a point mutation or an insertion\/deletion in a well-defined genetic region.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_column_10 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_group_1 et_pb_group et-last-child et_pb_module et_flex_group et_pb_css_mix_blend_mode_passthrough\">\n<div class=\"et_pb_text_12 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h3 style=\"text-align:left;\"><strong>Global mutation analysis by NGS<\/strong><\/h3>\n<\/div><\/div>\n\n<div class=\"et_pb_text_13 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:left;\">When the objective is to identify a set of variations across a genome or large genetic regions, an <strong>NGS (Next Generation Sequencing) re-sequencing<\/strong> approach may be preferred.<\/p>\n<p>This approach allows for the simultaneous analysis of a large number of sequences and the identification of different forms of genetic variations. <\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_column_11 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_group_2 et_pb_group et-last-child et_pb_module et_flex_group et_pb_css_mix_blend_mode_passthrough\">\n<div class=\"et_pb_text_14 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h3 style=\"text-align:left;\"><strong>Targeted detection by PCR or qPCR<\/strong><\/h3>\n<\/div><\/div>\n\n<div class=\"et_pb_text_15 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:left;\">In certain cases, when the target sequence is already known and the precise location of the mutation is identified, <strong>PCR or qPCR<\/strong> based approaches can be used to confirm the presence of a genetic variation. <\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_section_3 et_pb_section et_section_regular et_flex_section\"><div class=\"et_pb_row_7 et_pb_row et_flex_row preset--module--divi-row--default\"><div class=\"et_pb_column_12 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\"><div class=\"et_pb_text_16 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align:center;\"><strong>Int\u00e9ress\u00e9 par notre offre ?<\/strong><\/h2>\n<\/div><\/div><div class=\"et_pb_text_17 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:center;\">Vous souhaitez des informations compl\u00e9mentaires ?<\/p>\n<\/div><\/div><div class=\"et_pb_module et_pb_button_module_wrapper et_pb_button_1_wrapper preset--module--divi-button--6hgv0h8r0g_wrapper\"><a class=\"et_pb_button_1 et_pb_button et_pb_bg_layout_dark et_pb_module et_block_module preset--module--divi-button--6hgv0h8r0g\" href=\"https:\/\/www.biomnigene.fr\/contact\/\" data-icon=\"$\">Nous contacter<\/a><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-617","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.5 - 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