{"id":595,"date":"2026-05-18T20:26:59","date_gmt":"2026-05-18T20:26:59","guid":{"rendered":"https:\/\/www.biomnigene.fr\/detect-or-quantify-a-gene\/"},"modified":"2026-09-10T12:57:35","modified_gmt":"2026-09-10T12:57:35","slug":"detect-or-quantify-a-gene","status":"publish","type":"page","link":"https:\/\/www.biomnigene.fr\/en\/detect-or-quantify-a-gene\/","title":{"rendered":"Detect or Quantify a Gene"},"content":{"rendered":"<div class=\"et_pb_section_0 et_pb_section et_section_regular et_flex_section\">\n<div class=\"et_pb_row_0 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_0 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n\n\n\n\n\n<div class=\"et_pb_module et_d4_element dsm_breadcrumbs dsm_breadcrumbs_0\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module_inner\">\n\t\t\t\t\t<nav class=\"dsm_breadcrumbs_wrap\">\n\t\t\t\t<ul class=\"dsm_breadcrumbs\" itemscope itemtype=\"https:\/\/schema.org\/BreadcrumbList\">\t\t\t<li class=\"dsm_breadcrumbs_item dsm_home_item dsm_home_item_custom\" itemprop=\"itemListElement\" itemscope\n\t\t\titemtype=\"https:\/\/schema.org\/ListItem\">\n\t\t\t\t<a href=\"https:\/\/www.biomnigene.fr\/en\/\" itemprop=\"item\" target=\"\">\n\t\t\t\t\t<span class='dsm_home_text_wrapper'>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"et-pb-icon dsm_home_icon\">\ue074<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"dsm_home_text\" itemprop=\"name\">\n\t\t\t\t\t\t\t\t \t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t<\/a>\n\t\t\t\t<meta itemprop=\"position\" content=\"1\" \/>\n\t\t\t<\/li>\n\n\t\t<li class=\"dsm_breadcrumbs_separator\"> <span class=\"et-pb-icon dsm_separator_icon\">5<\/span><\/li><\/ul>\n\t\t\t<\/nav>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\n\n\n\n\n<\/div>\n<\/div>\n\n<div class=\"et_pb_row_1 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_1 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_0 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module preset--module--divi-text--default\"><div class=\"et_pb_text_inner\"><h1 style=\"text-align:center;\"><strong>Detect or Quantify a Gene<\/strong><\/h1>\n<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_section_1 et_pb_section et_section_regular et_flex_section\">\n<div class=\"et_pb_row_2 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_2 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_1 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>Identify the Presence of a Gene and Measure its Quantity<\/strong><\/h2>\n<\/div><\/div>\n<\/div>\n\n<div class=\"et_pb_column_3 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_2 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:left;\">The detection or quantification of a <strong>gene<\/strong> involves determining whether a specific genetic sequence is present in a sample and, in some cases, measuring the <strong>copy number of that sequence.<\/strong><\/p>\n<\/div><\/div>\n<\/div>\n\n<div class=\"et_pb_column_4 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_3 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:left;\">These analyses generally rely on <strong>gene amplification<\/strong> techniques, such as PCR or real-time quantitative PCR (qPCR), which allow for the detection of a target gene even when present in very low quantities.<\/p>\n<p>This approach is used to analyze genetic constructs, characterize biological samples, or measure gene expression under different experimental conditions.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_row_3 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_5 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_image_0 et_pb_image et_pb_module et_flex_module\"><span class=\"et_pb_image_wrap\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-determiner-ou-confirmer-la-sequence-d-un-gene-ou-d-un-genome-entier-illustration-11-2.webp\" width=\"1920\" height=\"640\" srcset=\"https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-determiner-ou-confirmer-la-sequence-d-un-gene-ou-d-un-genome-entier-illustration-11-2.webp 1920w, https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-determiner-ou-confirmer-la-sequence-d-un-gene-ou-d-un-genome-entier-illustration-11-2-1280x427.webp 1280w, https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-determiner-ou-confirmer-la-sequence-d-un-gene-ou-d-un-genome-entier-illustration-11-2-980x327.webp 980w, https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/img-determiner-ou-confirmer-la-sequence-d-un-gene-ou-d-un-genome-entier-illustration-11-2-480x160.webp 480w\" sizes=\"(min-width: 0px) and (max-width: 480px) 480px, (min-width: 481px) and (max-width: 980px) 980px, (min-width: 981px) and (max-width: 1280px) 1280px, (min-width: 1281px) 1920px, 100vw\" class=\"wp-image-266\" \/><\/span><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_row_4 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_6 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_12_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_4 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>When to Detect or Quantify a Gene?<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_5 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:left;\">Gene detection or quantification may be necessary in several situations, for example, to:<\/p>\n<ul>\n<li>verify the presence of a target gene in a genetic construct (plasmid, genomic DNA, etc.),<\/li>\n<li>identify a specific gene fragment in a sample,<\/li>\n<li>measure the copy number of a gene in a given sample,<\/li>\n<li>compare the presence or abundance of a gene across multiple samples.<\/li>\n<\/ul>\n<p>These analyses provide reliable information on the presence or quantity of a genetic sequence in a biological system.<\/p>\n<\/div><\/div>\n\n<div class=\"et_pb_module et_pb_button_module_wrapper et_pb_button_0_wrapper preset--module--divi-button--ssmr0y6bs7_wrapper\"><a class=\"et_pb_button_0 et_pb_button et_pb_bg_layout_dark et_pb_module et_block_module preset--module--divi-button--ssmr0y6bs7\" href=\"https:\/\/www.biomnigene.fr\/contact\/\" data-icon=\"$\">Contact Us<\/a><\/div>\n<\/div>\n\n<div class=\"et_pb_column_7 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_12_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_6 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>What the Analysis Enables<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_7 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:left;\">Gene detection and quantification provide essential information for many molecular biology studies.<\/p>\n<p>They notably allow for:<\/p>\n<ul>\n<li>confirming the presence of a gene or target sequence,<\/li>\n<li>measuring the amount of DNA corresponding to that gene in a sample,<\/li>\n<li>comparing multiple samples with each other,<\/li>\n<li>analyzing the variation in gene expression under experimental conditions.<\/li>\n<\/ul>\n<p>Quantitative PCR (qPCR), for example, allows for monitoring the amplification of a target sequence and inferring the initial quantity of DNA or RNA present in the sample.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_section_2 et_pb_section et_section_regular et_flex_section\">\n<div class=\"et_pb_row_5 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_8 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_8 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: center;\"><strong>Which Approaches for Gene Detection or Quantification?<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_9 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:center;\">The choice of method depends on the objective of the analysis and the type of data sought.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_row_6 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_9 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_10 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h3 style=\"text-align: left;\"><strong>Detection by PCR<\/strong><\/h3>\n<\/div><\/div>\n\n<div class=\"et_pb_text_11 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:left;\"><strong>Conventional PCR<\/strong> allows for the detection of a specific genetic sequence in a sample.<\/p>\n<p>This approach is particularly useful for verifying the presence or absence of a target gene in a genetic construct or genomic DNA.<\/p>\n<\/div><\/div>\n<\/div>\n\n<div class=\"et_pb_column_10 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_12 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h3 style=\"text-align: left;\"><strong>Quantification by Real-Time PCR (qPCR)<\/strong><\/h3>\n<\/div><\/div>\n\n<div class=\"et_pb_text_13 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:left;\"><strong>Real-time quantitative PCR<\/strong> allows for measuring the quantity of a target genetic sequence in a sample.<\/p>\n<p>By monitoring DNA amplification during the reaction, it is possible to estimate the <strong>copy number of a gene<\/strong> or analyze its <strong>expression level<\/strong> under different conditions. <\/p>\n<\/div><\/div>\n<\/div>\n\n<div class=\"et_pb_column_11 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_8_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_14 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_section_3 et_pb_section et_section_regular et_flex_section\">\n<div class=\"et_pb_row_7 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_12 et_pb_column et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_12_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_15 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>Choosing the Right Strategy for Your Project<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_16 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:left;\">The analysis strategy depends on several parameters:<\/p>\n<ul>\n<li>the nature of the sample analyzed,<\/li>\n<li>the objective of the study (simple detection or quantification),<\/li>\n<li>the expected level of precision.<\/li>\n<\/ul>\n<p>Upfront consideration helps define the most suitable approach to obtain reliable and interpretable results.<\/p>\n<\/div><\/div>\n<\/div>\n\n<div class=\"et_pb_column_13 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_12_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_17 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: left;\"><strong>Support at Every Step<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_18 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:left;\">Each project has specific technical requirements.<br \/>BIOMNIGENE supports its clients <strong>in implementing PCR and qPCR analyses<\/strong> to detect or quantify a target gene in different types of samples.<\/p>\n<p>This support enables the implementation of the most relevant experimental strategy and the acquisition of exploitable data for the remainder of the project.<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_section_4 et_pb_section et_section_regular et_flex_section\">\n<div class=\"et_pb_row_8 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_14 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_24_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_text_19 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><h2 style=\"text-align: center;\"><strong>Other Needs Related to Genetic Analysis<\/strong><\/h2>\n<\/div><\/div>\n\n<div class=\"et_pb_text_20 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:center;\">Gene detection and quantification are part of a broader set of genetic analyses, including:<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n<div class=\"et_pb_row_9 et_pb_row et_flex_row preset--module--divi-row--default\">\n<div class=\"et_pb_column_15 et_pb_column et-last-child et_flex_column et_pb_css_mix_blend_mode_passthrough et_flex_column_24_24 et_flex_column_12_24_tablet et_flex_column_24_24_phone\">\n<div class=\"et_pb_group_0 et_pb_group et_pb_module et_flex_group et_pb_css_mix_blend_mode_passthrough\">\n<div class=\"et_pb_image_1 et_pb_image et_pb_module et_flex_module\"><span class=\"et_pb_image_wrap\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/arrow-bleu.webp\" title=\"arrow-bleu\" width=\"80\" height=\"80\" srcset=\"https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/arrow-bleu.webp 80w\" sizes=\"(max-width: 80px) 100vw, 80px\" class=\"wp-image-259\" \/><\/span><\/div>\n\n<div class=\"et_pb_text_21 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:left;\"><strong>determining or confirming the sequence of a gene or an entire genome<\/strong><\/p>\n<\/div><\/div>\n<\/div>\n\n<div class=\"et_pb_group_1 et_pb_group et_pb_module et_flex_group et_pb_css_mix_blend_mode_passthrough\">\n<div class=\"et_pb_image_2 et_pb_image et_pb_module et_flex_module\"><span class=\"et_pb_image_wrap\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/arrow-bleu.webp\" title=\"arrow-bleu\" width=\"80\" height=\"80\" srcset=\"https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/arrow-bleu.webp 80w\" sizes=\"(max-width: 80px) 100vw, 80px\" class=\"wp-image-259\" \/><\/span><\/div>\n\n<div class=\"et_pb_text_22 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:left;\"><strong>searching for mutations, insertions, or deletions<\/strong><\/p>\n<\/div><\/div>\n<\/div>\n\n<div class=\"et_pb_group_2 et_pb_group et_pb_module et_flex_group et_pb_css_mix_blend_mode_passthrough\">\n<div class=\"et_pb_image_3 et_pb_image et_pb_module et_flex_module\"><span class=\"et_pb_image_wrap\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/arrow-bleu.webp\" title=\"arrow-bleu\" width=\"80\" height=\"80\" srcset=\"https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/arrow-bleu.webp 80w\" sizes=\"(max-width: 80px) 100vw, 80px\" class=\"wp-image-259\" \/><\/span><\/div>\n\n<div class=\"et_pb_text_23 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:left;\"><strong>studying gene expression<\/strong><\/p>\n<\/div><\/div>\n<\/div>\n\n<div class=\"et_pb_group_3 et_pb_group et-last-child et_pb_module et_flex_group et_pb_css_mix_blend_mode_passthrough\">\n<div class=\"et_pb_image_4 et_pb_image et_pb_module et_flex_module\"><span class=\"et_pb_image_wrap\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/arrow-bleu.webp\" title=\"arrow-bleu\" width=\"80\" height=\"80\" srcset=\"https:\/\/www.biomnigene.fr\/wp-content\/uploads\/2026\/05\/arrow-bleu.webp 80w\" sizes=\"(max-width: 80px) 100vw, 80px\" class=\"wp-image-259\" \/><\/span><\/div>\n\n<div class=\"et_pb_text_24 et_pb_text et_pb_bg_layout_light et_pb_module et_flex_module\"><div class=\"et_pb_text_inner\"><p style=\"text-align:left;\"><strong>performing genotyping analyses<\/strong><\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":0,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-595","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.5 - 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